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compound 4 octyl itaconate  (MedChemExpress)


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    MedChemExpress compound 4 octyl itaconate
    Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented <t>with</t> <t>4-octyl</t> itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.
    Compound 4 Octyl Itaconate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/compound+4+octyl+itaconate/4-Octyl+itaconate/pmc13106626-29-1-9
    Average 97 stars, based on 98 article reviews
    compound 4 octyl itaconate - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress"

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-43821-0

    Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented with 4-octyl itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.
    Figure Legend Snippet: Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented with 4-octyl itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.

    Techniques Used: Concentration Assay, Western Blot, Comparison

    Related Articles

    Concentration Assay:

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress
    Article Snippet: Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress.
    Article Snippet: Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.

    Western Blot:

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress
    Article Snippet: Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress.
    Article Snippet: Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.

    Comparison:

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress
    Article Snippet: Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress.
    Article Snippet: Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.Materials and Methods Reagents Lipopolysaccharide (LPS, 055:B5, #L2880) was acquired from ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S Sigma located in St. Louis, Missouri, USA.. The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.. ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.ELISA kits designed for assessing mouse tumor necrosis factor-α (TNF-α, #EMC102a) and interleukin-6 (IL-6, #EMC004.96) were obtained from Neobioscience Technology in Shenzhen, China.



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    compound 4 octyl itaconate  (MedChemExpress)
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    MedChemExpress compound 4 octyl itaconate
    Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented <t>with</t> <t>4-octyl</t> itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.
    Compound 4 Octyl Itaconate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 4. Increased succinate levels impair mitochondrial function in adrenocortical cells. (A,B) Succinate and fumarate levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in adrenal gland explants (A) and NCI-H295R cells (B) treated with dimethyl malonate (DMM) or diethyl succinate (DES) for 24 hr (n=5 for (A) and n=4 for (B)). (C) Oxygen consumption rate (OCR) measurement with Seahorse technology in NCI- H295R cells treated with DMM or DES for 24 hr (n=6). (D) Measurement of ATP/ADP ratio in NCI-H295R cells treated with DMM or DES for 24 hr (n=4– 12). (E,F) TMRE and Mitotracker Green FM staining assessed by flow cytometry in NCI-H295R cells treated with DES for 4 hr, MFI is shown (n=7 for (E) and n=4, one from two experiments for (F)). (G) ROS measurement in NCI-H295R cells treated with DMM or DES for 2 hr (n=10–12). (H) Measurement of NADPH/NADP+ ratio in NCI-H295R cells treated with DMM for 24 hr (n=3–4). (I) Isocitrate levels measured by LC-MS/MS in NCI-H295R cells treated for 24 hr with <t>AG221</t> or DMSO (n=6). (J) OCR measurement in NCI-H295R cells treated for 24 hr with AG221 or DMSO (n=10). (K) TMRE staining and flow cytometry in NCI-H295R cells treated for 4 hr with AG221 or DMSO, MFI is shown (n=7). Data in (A–B,D–I,K) are presented as mean ± s.d. Data in (C,J) are presented as mean ± s.e.m. Statistical analysis was done with one-way ANOVA (A, G) or two-tailed (B,D,E,F,I,K) or one-tailed (H) Mann-Whitney test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Drug Enasidenib Ag221 Selleckchem S8205 Chemical Compound, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented with 4-octyl itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.

    Journal: Scientific Reports

    Article Title: Protective role of IRG1/itaconate in acute myocardial injury: association with NLRP3 inflammasome and oxidative stress

    doi: 10.1038/s41598-026-43821-0

    Figure Lengend Snippet: Nrf2 pathway and oxidative stress were associated with IRG1/itaconate in acute myocardial injury. WT or IRG1 KO mice with acute myocardial injury were supplemented with 4-octyl itaconate (4-OI) and sacrificed 18 h post LPS exposure. ( A – D ) The levels of ROS, TBARS, the GSSG/GSH ratioin cardiac tissue homogenates and serum 8-OH-dG concentration were quantified ( n = 8). ( E , F ) Representative western blots and statistical analysis of IL-1β, NLRP3, Keap1, Nrf2, HO-1 and NQO1in heart samples were displayed ( n = 4). Data are representative of two independent experiments and show mean ± SD. ( A – D ) Two-way ANOVA with Sidak multiple comparison test. ( F ) two-sided t -test.

    Article Snippet: The compound 4-Octyl itaconate (4-OI, #HY-112675) was sourced from MedChemExpress, USA.

    Techniques: Concentration Assay, Western Blot, Comparison

    Figure 4. Increased succinate levels impair mitochondrial function in adrenocortical cells. (A,B) Succinate and fumarate levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in adrenal gland explants (A) and NCI-H295R cells (B) treated with dimethyl malonate (DMM) or diethyl succinate (DES) for 24 hr (n=5 for (A) and n=4 for (B)). (C) Oxygen consumption rate (OCR) measurement with Seahorse technology in NCI- H295R cells treated with DMM or DES for 24 hr (n=6). (D) Measurement of ATP/ADP ratio in NCI-H295R cells treated with DMM or DES for 24 hr (n=4– 12). (E,F) TMRE and Mitotracker Green FM staining assessed by flow cytometry in NCI-H295R cells treated with DES for 4 hr, MFI is shown (n=7 for (E) and n=4, one from two experiments for (F)). (G) ROS measurement in NCI-H295R cells treated with DMM or DES for 2 hr (n=10–12). (H) Measurement of NADPH/NADP+ ratio in NCI-H295R cells treated with DMM for 24 hr (n=3–4). (I) Isocitrate levels measured by LC-MS/MS in NCI-H295R cells treated for 24 hr with AG221 or DMSO (n=6). (J) OCR measurement in NCI-H295R cells treated for 24 hr with AG221 or DMSO (n=10). (K) TMRE staining and flow cytometry in NCI-H295R cells treated for 4 hr with AG221 or DMSO, MFI is shown (n=7). Data in (A–B,D–I,K) are presented as mean ± s.d. Data in (C,J) are presented as mean ± s.e.m. Statistical analysis was done with one-way ANOVA (A, G) or two-tailed (B,D,E,F,I,K) or one-tailed (H) Mann-Whitney test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: eLife

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction

    doi: 10.7554/elife.83064

    Figure Lengend Snippet: Figure 4. Increased succinate levels impair mitochondrial function in adrenocortical cells. (A,B) Succinate and fumarate levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in adrenal gland explants (A) and NCI-H295R cells (B) treated with dimethyl malonate (DMM) or diethyl succinate (DES) for 24 hr (n=5 for (A) and n=4 for (B)). (C) Oxygen consumption rate (OCR) measurement with Seahorse technology in NCI- H295R cells treated with DMM or DES for 24 hr (n=6). (D) Measurement of ATP/ADP ratio in NCI-H295R cells treated with DMM or DES for 24 hr (n=4– 12). (E,F) TMRE and Mitotracker Green FM staining assessed by flow cytometry in NCI-H295R cells treated with DES for 4 hr, MFI is shown (n=7 for (E) and n=4, one from two experiments for (F)). (G) ROS measurement in NCI-H295R cells treated with DMM or DES for 2 hr (n=10–12). (H) Measurement of NADPH/NADP+ ratio in NCI-H295R cells treated with DMM for 24 hr (n=3–4). (I) Isocitrate levels measured by LC-MS/MS in NCI-H295R cells treated for 24 hr with AG221 or DMSO (n=6). (J) OCR measurement in NCI-H295R cells treated for 24 hr with AG221 or DMSO (n=10). (K) TMRE staining and flow cytometry in NCI-H295R cells treated for 4 hr with AG221 or DMSO, MFI is shown (n=7). Data in (A–B,D–I,K) are presented as mean ± s.d. Data in (C,J) are presented as mean ± s.e.m. Statistical analysis was done with one-way ANOVA (A, G) or two-tailed (B,D,E,F,I,K) or one-tailed (H) Mann-Whitney test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

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    Techniques: Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Staining, Flow Cytometry, Two Tailed Test, One-tailed Test, MANN-WHITNEY